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Registros recuperados : 25 | |
1. | | MADRUGA, C. R.; LEAL, C. R. B.; MACHADO, R. Z.; MIGUITA, M.; ARAUJO, F. R.; KESSLER, R. H. Caracterizacao antigenica dos isolados de Babesia bovis e Babesia bigemina provenientes do Rio Grande do Sul. In : SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 10.; SEMINARIO DE PARASITOLOGIA VETERINARIA DO MERCOSUL, 1., 1997, Itajai. Anais... Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.6, n.2, p. 306, ago. 1997. Suplemento 1. Resumo P9. CNPGC. Biblioteca(s): Embrapa Gado de Corte. |
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4. | | GIGLIOTI, R.; BILHASSI, T. B.; MACHADO, R. Z.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. Repetibilidade e associação entre técnicas moleculares e sorológicas quantitativas na detecção de babesia bovis em bovinos de corte criados no estado de São Paulo, Brasil. In: CONGRESO DE LA ASOCIACIÓN LATINO AMERICANA, 24.; CONGRESO DE LA SOCIED CHILENA DE PRODUCIÓN ANIMAL, 40., 2015, Puerto Varas. Anales... Puerto Varas: ALPA, 2015. 1 CD-ROM Biblioteca(s): Embrapa Pecuária Sudeste. |
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5. | | PATELLI, T. H. C.; MARQUES, L. C.; MACHADO, R. Z.; OLIVEIRA, T. M. F. S.; PASSOS, P. B. Resposta imunológica humoral de caprinos experimentalmente infectados com Trypanosoma evansi (Sarcomastigophora: Trypanosomatidae). ARS Veterinária, Jaboticabal, v. 23, n. 3, p. 146-150, 2007. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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6. | | ALMEIDA, K. de S.; COSTA, A. F.; SILVA, P. C. da; FAGLIARI, J. J.; MACHADO, R. Z.; NASCIMENTO, A. A. do. Acute phase proteins: a potential approach for diagnosing chronic infection by Trypanosoma vivax. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 21, n. 2, p. 97-100, abr./jun. 2012. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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7. | | MORAES, L. M. de B.; RAIMUNDO, J. M.; GUIMARÃES, A.; SANTOS, H. A.; MACEDO JÚNIOR, G. de L.; MASSARD, C. L.; MACHADO, R. Z.; BALDANI, C. D. Occurrence of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies in goats and sheep in western Maranhão, Brazil. Revista Brasileira de Parasitolologia Veterinária, Jaboticabal, v. 20, n. 4, p. 312-317, out./dez. 2011. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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8. | | GUIMARÃES, A.; RAIMUNDO, J. M.; MORAES, L M. B.; SILVA, A. T.; SANTOS, H. A.; PIRES, M. S.; MACHADO, R. Z.; BALDANI, C. D. Occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from four districts of Tocantins state, Brazilian Amazon Region. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n.2, p.110-114, fev. 2015. Biblioteca(s): Embrapa Unidades Centrais. |
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9. | | GUIMARÃES, A.; RAIMUNDO, J. M.; MORAES, L. M. B.; SILVA, A. T.; SANTOS, H. A.; PIRES, M. S.; MACHADO, R. Z.; BALDANI, C. D. Ocorrência de anticorpos anti-Toxoplasma gondii e anti-Neospora caninum em ovelhas de quatro municípios do estado do Tocantins, Região da Amazônia Legal Brasileira. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 2, p. 110-114, fev. 2015. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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10. | | GIGLIOTI, R.; BILHASSI, T. B.; SANTANA, C. H.; PORTILHO, A. I.; MACHADO, R. Z.; OLIVEIRA, H. N. de; MARCONDES, C. R.; OLIVEIRA, M. C. de S. Correlação entre os níveis de infecção por Babesia bovis, Babesia bigemina e Anaplasma marginale determinados por qPCR e infestações por Rhipicephalus microplus em fêmeas Canchim. In: CONGRESO DA LA ASOCIACIÓN LATINOAMERICANA DE PRODUCCIÓN ANIMAL, 25., 2016, Pernambuco. Anais... Pernambuco: SNPA, 2016. Biblioteca(s): Embrapa Pecuária Sudeste. |
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11. | | JULIANO, R. S.; FIORAVANTI, M. C. S.; MACHADO, R. Z.; ABREU, U. G. P. de; BARINI, A. C.; BORGES, A. C.; PAULA NETO, J. B.; TRINDADE, B. R. Hematologia e bioquímica sérica de bovinos curraleiro pé duro infectados por Babesia spp. e Leptospira spp. Corumbá: Embrapa Pantanal, 2017. 16 p. (Embrapa Pantanal. Boletim de Pesquisa e Desenvolvimento, 137). Biblioteca(s): Embrapa Pantanal. |
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12. | | FREITAS, F. L. da; ALMEIDA, K. de S.; NASCIMENTO, A. A. do; MACHADO, C. R.; VESCHI, J. L. A. MACHADO, R. Z. Espécies do gênero Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) em caprinos leiteiros mantidos em sistema intensivo na região de São José do Rio Preto, estado de São Paulo, Brasil. Revista Brasileira de Parasitologia Veterinária, v. 14, n. 1, p. 7-10, 2005. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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13. | | RAMOS, R. A. do N.; RAMOS, C. A. do N.; JUSI, M. M. G.; ARAUJO, F. R.; MACHADO, R. Z.; FAUSTINO, M. A. da G.; ALVES, L. C. Polymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs. Revista Brasileira de Parasitologia Veterinária, v. 21, n. 3, p. 192-195, jul.-set. 2012 Biblioteca(s): Embrapa Gado de Corte. |
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14. | | JULIANO, R. S.; FIORAVANTI, M. C. S.; SERENO, J. R. B.; ABREU, U. G. P. de; JAYME, V. de S.; SILVA, A. C.; MACHADO, R. Z.; BRITTO, W. M. E. D.; ALFIERI, A. A.; SANTOS, S. A. Aspectos sanitários dos núcleos de conservação in situ de bovinos pantaneiros. Corumbá: Embrapa Pantanal, 2011. 16 p. (Embrapa Pantanal. Boletim de Pesquisa e Desenvolvimento, 103). Biblioteca(s): Embrapa Pantanal. |
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15. | | OBREGÓN, D.; CORONA, B. G.; FUENTE, J. de la; CABEZAS-CRUZ, A.; GONÇALVES, L. R.; MATOS, C. A.; ARMAS, Y.; HINOJOSA, Y.; ALFONSO, P.; OLIVEIRA, M. C. de S.; MACHADO, R. Z. Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba. Veterinary Parasitology, v. 13, p. 180-187, 2018. Biblioteca(s): Embrapa Pecuária Sudeste. |
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16. | | GIGLIOTI, R.; OLIVEIRA, H. N.; IBELLI, A. M. G.; BILHASSI, T. B.; NEO, T. A.; SANTANA, C. H.; RABELO, M. D.; MACHADO, R. Z.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S. Neither quantification by qPCR nor quantitative Elisa can be used to discriminate angus cattle for resistance/susceptibility to Babesia bovis. Ticks and Tick-borne Diseases, v. 8, n. 3, p. 335-340, 2017. Biblioteca(s): Embrapa Pecuária Sudeste. |
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17. | | MOREIRA, C. T.; SOUZA, P. I. M.; LIMA, C. A; FRANÇA NETO, J. de B.; ABUD, S.; TEIXEIRA, R. N.; SANTOS, R. L. B.; MACHADO, R. Z.; MONTEIRO, P. M. F. O.; NUNES JUNIOR, J.; GUERZONI, R. A. Ocorrência de variação na cor do hilo nas cultivares de soja BRSMS Taquari e BRSGO Santa Cruz. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 25., 2003, Uberaba. Resumos... Londrina: Embrapa Soja: EPAMIG: Fundação Triângulo, 2003. p. 306. (Embrapa Soja. Documentos, 209). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite. Biblioteca(s): Embrapa Soja. |
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18. | | GIGLIOTI, A.; OLIVEIRA, H. N.; SANTANA, C. H.; IBELLI, A. M. G.; NÉO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. Ticks and Tick-borne Diseases, v. 7, p. 657-66, 2016. Biblioteca(s): Embrapa Rondônia. |
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19. | | GIGLIOTI, R.; OLIVEIRA, H. N. de; SANTANA, C. H.; IBELLI, A. M. G.; NEO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. Ticks and Tick-borne Diseases, v. 7, n. 5, p. 657-662, jul. 2016. Biblioteca(s): Embrapa Pecuária Sudeste. |
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20. | | OBREGÓN, D.; CABEZAS-CRUZ, A.; ARMAS, Y.; SILVA, J. B.; FONSECA, A. H.; ANDRÉ, M. R.; ALFONSO, P.; OLIVEIRA, M. C. de S.; MACHADO, R. Z.; CORONA-GONZÁLEZ, B. High co-infection rates of Babesia bovis, Babesia bigemina, and Anaplasma marginale in water buffalo in Western Cuba. Parasitology Research, v. 118, n. 3, p.955?967, 2019. Biblioteca(s): Embrapa Pecuária Sudeste. |
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Registros recuperados : 25 | |
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Registro Completo
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
03/08/2016 |
Data da última atualização: |
07/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
GIGLIOTI, A.; OLIVEIRA, H. N.; SANTANA, C. H.; IBELLI, A. M. G.; NÉO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. |
Afiliação: |
Unesp (Jaboticabal); Unesp (Jaboticabal); Unesp (Jaboticabal); Unesp (Jaboticabal); UFSCAR; Unesp (Jaboticabal); MARCIO DIAS RABELO, CPPSE; Unesp (Jaboticabal); LUCIANA GATTO BRITO, CPAF-Rondonia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 7, p. 657-66, 2016. |
Idioma: |
Inglês |
Conteúdo: |
The levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. MenosThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus a... Mostrar Tudo |
Palavras-Chave: |
QPCR; R microplus; Resistance. |
Thesaurus NAL: |
babesiosis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03159naa a2200277 a 4500 001 2050164 005 2022-03-07 008 2016 bl uuuu u00u1 u #d 100 1 $aGIGLIOTI, A. 245 $aBabesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil.$h[electronic resource] 260 $c2016 520 $aThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. 650 $ababesiosis 653 $aQPCR 653 $aR microplus 653 $aResistance 700 1 $aOLIVEIRA, H. N. 700 1 $aSANTANA, C. H. 700 1 $aIBELLI, A. M. G. 700 1 $aNÉO, T. A. 700 1 $aBILHASSI, T. B. 700 1 $aRABELO, M. D. 700 1 $aMACHADO, R. Z. 700 1 $aBRITO, L. G. 700 1 $aOLIVEIRA, M. C. de S. 773 $tTicks and Tick-borne Diseases$gv. 7, p. 657-66, 2016.
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